POS0429 ACTIVATED RHEUMATOID ARTHRITIS SYNOVIAL FIBROBLASTS ALTER OSTEOCLAST DIFFERENTIATION AND ACTIVITY
نویسندگان
چکیده
Background In rheumatoid arthritis (RA), osteoclasts are one of the most important mediators bone erosion. addition, RA synovial fibroblasts (RASF) have a major influence on joint erosion in RA. They affect osteoclast differentiation e.g. by increased RANKL production or secretion other pro-osteoclastogenic factors such as IL-6. addition to effect soluble and IL-1, visfatin inhibits differentiation. Furthermore, increases pro-inflammatory RASF, IL-6 matrix degrading enzymes. Objectives this study, RASF with/without activation IL-1 osteoclastogenesis was evaluated. Methods Blood from healthy donors patients used for PBMC isolation. RANKL, TGF-β hM-CSF were added induce RASF-conditioned media (CM) prepared confluent cultured 48h. Differentiating PBMCs monoculture compared with CM (CM: 10%, 20%, 30%) well direct co-culture stimulation (0.05ng/ml), (25ng/ml). After two weeks culture, cells stained using TRAP staining. 3-5 images per quantification dependent variability wells. measured ELISA supernatants collected at day 14. Results (e.g. co-culture: 2,8-fold) (CM-visfatin: 10%=4,3-fold, 20%=5,4-fold, 30%=4,2-fold; 9,5-fold) unstimulated control all settings. controls (healthy 30%: p=0.0342, p=0.0133, p=0,0133; RA: n=3 each). Of note, baseline concentrations higher donors. Co-culture showed an additional increase levels settings monoculture: 4.71±5.75pg/ml, 141.09±182.79pg/ml; 7241±10398pg/ml, 24535±16994pg/ml;). During differentiation, similar proportion 2 vs. numbers nuclei cell control. coculture stronger signal especially stimulated co-cultures. Conclusion Both, coculture, control, whereas The containing secreted did not prominent osteoclastogenesis. However, presence showing activity differentiated co-cultures but visfatin. References None. Disclosure Interests None declared.
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ژورنال
عنوان ژورنال: Annals of the Rheumatic Diseases
سال: 2022
ISSN: ['1468-2060', '0003-4967']
DOI: https://doi.org/10.1136/annrheumdis-2022-eular.3135